Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM03-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM03-Larvae_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 48379049 Number of paired-end alignments with a unique best hit: 15618590 Mapping efficiency: 32.3% Sequence pairs with no alignments under any condition: 25139522 Sequence pairs did not map uniquely: 7620937 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3745895 ((converted) top strand) GA/CT/CT: 4059444 (complementary to (converted) top strand) GA/CT/GA: 4067743 (complementary to (converted) bottom strand) CT/GA/GA: 3745502 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 516811610 Total methylated C's in CpG context: 13655464 Total methylated C's in CHG context: 1514430 Total methylated C's in CHH context: 8653755 Total methylated C's in Unknown context: 247522 Total unmethylated C's in CpG context: 59306412 Total unmethylated C's in CHG context: 103483679 Total unmethylated C's in CHH context: 330197870 Total unmethylated C's in Unknown context: 2071696 C methylated in CpG context: 18.7% C methylated in CHG context: 1.4% C methylated in CHH context: 2.6% C methylated in unknown context (CN or CHN): 10.7% Bismark completed in 0d 2h 44m 11s