Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM03-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM03-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 48379049
Paired-end alignments with a unique best hit 15618590
Pairs without alignments under any condition 25139522
Pairs that did not map uniquely 7620937
Genomic sequence context not extractable (edges of chromosomes) 6

Cytosine Methylation

Total C's analysed 516811610
Methylated C's in CpG context 13655464
Methylated C's in CHG context 1514430
Methylated C's in CHH context 8653755
Methylated C's in Unknown context 247522
Unmethylated C's in CpG context 59306412
Unmethylated C's in CHG context 103483679
Unmethylated C's in CHH context 330197870
Unmethylated C's in Unknown context 2071696
Percentage methylation (CpG context) 18.7%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 2.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3745895 original top strand
CTOT 4059444 complementary to original top strand
CTOB 4067743 complementary to original bottom strand
OB 3745502 original bottom strand

Deduplication

Alignments analysed 15618584
Unique alignments 11405235
Duplicates removed 4213349
Duplicated alignments were found at 3008884 different positions

Cytosine Methylation after Extraction

Total C's analysed 217099541
Methylated C's in CpG context 5580527
Methylated C's in CHG context 650121
Methylated C's in CHH context 3495169
Unmethylated C's in CpG context 24851645
Unmethylated C's in CHG context 43074617
Unmethylated C's in CHH context 139447462
Percentage methylation (CpG context) 18.3%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.4%

M-Bias Plot