Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM01-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM01-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 75480641 Number of paired-end alignments with a unique best hit: 8248837 Mapping efficiency: 10.9% Sequence pairs with no alignments under any condition: 61730915 Sequence pairs did not map uniquely: 5500889 Sequence pairs which were discarded because genomic sequence could not be extracted: 9 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2410845 ((converted) top strand) GA/CT/CT: 1743331 (complementary to (converted) top strand) GA/CT/GA: 1725475 (complementary to (converted) bottom strand) CT/GA/GA: 2369177 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 171219488 Total methylated C's in CpG context: 3194441 Total methylated C's in CHG context: 407198 Total methylated C's in CHH context: 8271861 Total methylated C's in Unknown context: 242482 Total unmethylated C's in CpG context: 16796262 Total unmethylated C's in CHG context: 28691213 Total unmethylated C's in CHH context: 113858513 Total unmethylated C's in Unknown context: 978439 C methylated in CpG context: 16.0% C methylated in CHG context: 1.4% C methylated in CHH context: 6.8% C methylated in unknown context (CN or CHN): 19.9% Bismark completed in 0d 2h 22m 45s