Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM01-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF07-EM01-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 75480641
Paired-end alignments with a unique best hit 8248837
Pairs without alignments under any condition 61730915
Pairs that did not map uniquely 5500889
Genomic sequence context not extractable (edges of chromosomes) 9

Cytosine Methylation

Total C's analysed 171219488
Methylated C's in CpG context 3194441
Methylated C's in CHG context 407198
Methylated C's in CHH context 8271861
Methylated C's in Unknown context 242482
Unmethylated C's in CpG context 16796262
Unmethylated C's in CHG context 28691213
Unmethylated C's in CHH context 113858513
Unmethylated C's in Unknown context 978439
Percentage methylation (CpG context) 16.0%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 6.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2410845 original top strand
CTOT 1743331 complementary to original top strand
CTOB 1725475 complementary to original bottom strand
OB 2369177 original bottom strand

Deduplication

Alignments analysed 8248828
Unique alignments 4977458
Duplicates removed 3271370
Duplicated alignments were found at 1767598 different positions

Cytosine Methylation after Extraction

Total C's analysed 57038120
Methylated C's in CpG context 1055701
Methylated C's in CHG context 122873
Methylated C's in CHH context 1904204
Unmethylated C's in CpG context 5660416
Unmethylated C's in CHG context 9636803
Unmethylated C's in CHH context 38658123
Percentage methylation (CpG context) 15.7%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 4.7%

M-Bias Plot