Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF06-EM06-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF06-EM06-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 80247899
Paired-end alignments with a unique best hit 21726849
Pairs without alignments under any condition 48022455
Pairs that did not map uniquely 10498595
Genomic sequence context not extractable (edges of chromosomes) 20

Cytosine Methylation

Total C's analysed 540906480
Methylated C's in CpG context 14456921
Methylated C's in CHG context 3822113
Methylated C's in CHH context 21997184
Methylated C's in Unknown context 525384
Unmethylated C's in CpG context 53245023
Unmethylated C's in CHG context 97253683
Unmethylated C's in CHH context 350131556
Unmethylated C's in Unknown context 3396653
Percentage methylation (CpG context) 21.4%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 5.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5390863 original top strand
CTOT 5508004 complementary to original top strand
CTOB 5470207 complementary to original bottom strand
OB 5357755 original bottom strand

Deduplication

Alignments analysed 21726829
Unique alignments 8138371
Duplicates removed 13588458
Duplicated alignments were found at 4440007 different positions

Cytosine Methylation after Extraction

Total C's analysed 119766527
Methylated C's in CpG context 2938477
Methylated C's in CHG context 820919
Methylated C's in CHH context 4945042
Unmethylated C's in CpG context 11487595
Unmethylated C's in CHG context 20543897
Unmethylated C's in CHH context 79030597
Percentage methylation (CpG context) 20.4%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 5.9%

M-Bias Plot