Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF06-EM01-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF06-EM01-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 56782240
Paired-end alignments with a unique best hit 19254185
Pairs without alignments under any condition 28725008
Pairs that did not map uniquely 8803047
Genomic sequence context not extractable (edges of chromosomes) 13

Cytosine Methylation

Total C's analysed 666787630
Methylated C's in CpG context 17066150
Methylated C's in CHG context 1883582
Methylated C's in CHH context 9735487
Methylated C's in Unknown context 310809
Unmethylated C's in CpG context 78528070
Unmethylated C's in CHG context 132143484
Unmethylated C's in CHH context 427430857
Unmethylated C's in Unknown context 2747140
Percentage methylation (CpG context) 17.9%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 2.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4541918 original top strand
CTOT 5077191 complementary to original top strand
CTOB 5090505 complementary to original bottom strand
OB 4544558 original bottom strand

Deduplication

Alignments analysed 19254172
Unique alignments 14750591
Duplicates removed 4503581
Duplicated alignments were found at 3431900 different positions

Cytosine Methylation after Extraction

Total C's analysed 299708571
Methylated C's in CpG context 7531004
Methylated C's in CHG context 886898
Methylated C's in CHH context 4401630
Unmethylated C's in CpG context 35161182
Unmethylated C's in CHG context 58901598
Unmethylated C's in CHH context 192826259
Percentage methylation (CpG context) 17.6%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.2%

M-Bias Plot