Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM06-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM06-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 62575164
Paired-end alignments with a unique best hit 21714872
Pairs without alignments under any condition 32866900
Pairs that did not map uniquely 7993392
Genomic sequence context not extractable (edges of chromosomes) 8

Cytosine Methylation

Total C's analysed 830731865
Methylated C's in CpG context 22400465
Methylated C's in CHG context 6818064
Methylated C's in CHH context 29976832
Methylated C's in Unknown context 454341
Unmethylated C's in CpG context 90547001
Unmethylated C's in CHG context 153083789
Unmethylated C's in CHH context 527905714
Unmethylated C's in Unknown context 3354289
Percentage methylation (CpG context) 19.8%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 5.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5154322 original top strand
CTOT 5691891 complementary to original top strand
CTOB 5710450 complementary to original bottom strand
OB 5158201 original bottom strand

Deduplication

Alignments analysed 21714864
Unique alignments 13459960
Duplicates removed 8254904
Duplicated alignments were found at 5003875 different positions

Cytosine Methylation after Extraction

Total C's analysed 333355883
Methylated C's in CpG context 8758572
Methylated C's in CHG context 2939623
Methylated C's in CHH context 12862135
Unmethylated C's in CpG context 35840966
Unmethylated C's in CHG context 59986409
Unmethylated C's in CHH context 212968178
Percentage methylation (CpG context) 19.6%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 5.7%

M-Bias Plot