Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM05-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM05-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 48548054
Paired-end alignments with a unique best hit 4410419
Pairs without alignments under any condition 40594655
Pairs that did not map uniquely 3542980
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 76674394
Methylated C's in CpG context 1156945
Methylated C's in CHG context 231352
Methylated C's in CHH context 4537735
Methylated C's in Unknown context 138959
Unmethylated C's in CpG context 7723284
Unmethylated C's in CHG context 12186707
Unmethylated C's in CHH context 50838371
Unmethylated C's in Unknown context 731315
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) 1.9%
Percentage methylation (CHH context) 8.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1139379 original top strand
CTOT 1074108 complementary to original top strand
CTOB 1066348 complementary to original bottom strand
OB 1130577 original bottom strand

Deduplication

Alignments analysed 4410412
Unique alignments 3199072
Duplicates removed 1211340
Duplicated alignments were found at 831028 different positions

Cytosine Methylation after Extraction

Total C's analysed 28591365
Methylated C's in CpG context 433213
Methylated C's in CHG context 84371
Methylated C's in CHH context 1439414
Unmethylated C's in CpG context 2905613
Unmethylated C's in CHG context 4576987
Unmethylated C's in CHH context 19151767
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) 1.8%
Percentage methylation (CHH context) 7.0%

M-Bias Plot