Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM01-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM01-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 39294742
Paired-end alignments with a unique best hit 11922548
Pairs without alignments under any condition 21460435
Pairs that did not map uniquely 5911759
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 399118445
Methylated C's in CpG context 11209649
Methylated C's in CHG context 1135547
Methylated C's in CHH context 6939128
Methylated C's in Unknown context 307446
Unmethylated C's in CpG context 48189502
Unmethylated C's in CHG context 82581213
Unmethylated C's in CHH context 249063406
Unmethylated C's in Unknown context 1774410
Percentage methylation (CpG context) 18.9%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 2.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2903527 original top strand
CTOT 3054689 complementary to original top strand
CTOB 3059526 complementary to original bottom strand
OB 2904803 original bottom strand

Deduplication

Alignments analysed 11922545
Unique alignments 8834392
Duplicates removed 3088153
Duplicated alignments were found at 2291120 different positions

Cytosine Methylation after Extraction

Total C's analysed 166834306
Methylated C's in CpG context 4617923
Methylated C's in CHG context 519586
Methylated C's in CHH context 3068644
Unmethylated C's in CpG context 20109284
Unmethylated C's in CHG context 34301616
Unmethylated C's in CHH context 104217253
Percentage methylation (CpG context) 18.7%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.9%

M-Bias Plot