Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF04-EM05-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF04-EM05-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 45628766
Paired-end alignments with a unique best hit 16508157
Pairs without alignments under any condition 21474728
Pairs that did not map uniquely 7645881
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 576961869
Methylated C's in CpG context 15192000
Methylated C's in CHG context 1474807
Methylated C's in CHH context 7395303
Methylated C's in Unknown context 261520
Unmethylated C's in CpG context 69576219
Unmethylated C's in CHG context 117554488
Unmethylated C's in CHH context 365769052
Unmethylated C's in Unknown context 2415126
Percentage methylation (CpG context) 17.9%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 2.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3903278 original top strand
CTOT 4347635 complementary to original top strand
CTOB 4353913 complementary to original bottom strand
OB 3903326 original bottom strand

Deduplication

Alignments analysed 16508152
Unique alignments 12215079
Duplicates removed 4293073
Duplicated alignments were found at 3177425 different positions

Cytosine Methylation after Extraction

Total C's analysed 245132023
Methylated C's in CpG context 6318934
Methylated C's in CHG context 647341
Methylated C's in CHH context 3224958
Unmethylated C's in CpG context 29453595
Unmethylated C's in CHG context 49546217
Unmethylated C's in CHH context 155940978
Percentage methylation (CpG context) 17.7%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 2.0%

M-Bias Plot