Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF03-EM04-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF03-EM04-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 74184423
Paired-end alignments with a unique best hit 23908336
Pairs without alignments under any condition 38192824
Pairs that did not map uniquely 12083263
Genomic sequence context not extractable (edges of chromosomes) 10

Cytosine Methylation

Total C's analysed 722086594
Methylated C's in CpG context 22718197
Methylated C's in CHG context 7003673
Methylated C's in CHH context 29868572
Methylated C's in Unknown context 687888
Unmethylated C's in CpG context 78620926
Unmethylated C's in CHG context 140604821
Unmethylated C's in CHH context 443270405
Unmethylated C's in Unknown context 3669282
Percentage methylation (CpG context) 22.4%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 6.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6549117 original top strand
CTOT 5400479 complementary to original top strand
CTOB 5409043 complementary to original bottom strand
OB 6549687 original bottom strand

Deduplication

Alignments analysed 23908326
Unique alignments 17883906
Duplicates removed 6024420
Duplicated alignments were found at 4501601 different positions

Cytosine Methylation after Extraction

Total C's analysed 295184891
Methylated C's in CpG context 9129077
Methylated C's in CHG context 2884396
Methylated C's in CHH context 12386503
Unmethylated C's in CpG context 31995225
Unmethylated C's in CHG context 57042151
Unmethylated C's in CHH context 181747539
Percentage methylation (CpG context) 22.2%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 6.4%

M-Bias Plot