Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF03-EM03-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF03-EM03-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 132787654
Paired-end alignments with a unique best hit 24296257
Pairs without alignments under any condition 94202406
Pairs that did not map uniquely 14288991
Genomic sequence context not extractable (edges of chromosomes) 6

Cytosine Methylation

Total C's analysed 511101837
Methylated C's in CpG context 12712115
Methylated C's in CHG context 3117783
Methylated C's in CHH context 26175201
Methylated C's in Unknown context 660360
Unmethylated C's in CpG context 46597944
Unmethylated C's in CHG context 86336289
Unmethylated C's in CHH context 336162505
Unmethylated C's in Unknown context 3082294
Percentage methylation (CpG context) 21.4%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 7.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6744796 original top strand
CTOT 5426945 complementary to original top strand
CTOB 5402692 complementary to original bottom strand
OB 6721818 original bottom strand

Deduplication

Alignments analysed 24296251
Unique alignments 16703527
Duplicates removed 7592724
Duplicated alignments were found at 4955338 different positions

Cytosine Methylation after Extraction

Total C's analysed 190049367
Methylated C's in CpG context 4738667
Methylated C's in CHG context 1177474
Methylated C's in CHH context 8891401
Unmethylated C's in CpG context 17482361
Unmethylated C's in CHG context 32176756
Unmethylated C's in CHH context 125582708
Percentage methylation (CpG context) 21.3%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 6.6%

M-Bias Plot