Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF02-EM02-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF02-EM02-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 56944147 Number of paired-end alignments with a unique best hit: 14177297 Mapping efficiency: 24.9% Sequence pairs with no alignments under any condition: 33479307 Sequence pairs did not map uniquely: 9287543 Sequence pairs which were discarded because genomic sequence could not be extracted: 18 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3991436 ((converted) top strand) GA/CT/CT: 3145292 (complementary to (converted) top strand) GA/CT/GA: 3103611 (complementary to (converted) bottom strand) CT/GA/GA: 3936940 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 368303412 Total methylated C's in CpG context: 8219994 Total methylated C's in CHG context: 1030273 Total methylated C's in CHH context: 9468397 Total methylated C's in Unknown context: 343493 Total unmethylated C's in CpG context: 43793710 Total unmethylated C's in CHG context: 72295531 Total unmethylated C's in CHH context: 233495507 Total unmethylated C's in Unknown context: 2399471 C methylated in CpG context: 15.8% C methylated in CHG context: 1.4% C methylated in CHH context: 3.9% C methylated in unknown context (CN or CHN): 12.5% Bismark completed in 0d 2h 30m 25s