Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF02-EM02-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF02-EM02-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 56944147
Paired-end alignments with a unique best hit 14177297
Pairs without alignments under any condition 33479307
Pairs that did not map uniquely 9287543
Genomic sequence context not extractable (edges of chromosomes) 18

Cytosine Methylation

Total C's analysed 368303412
Methylated C's in CpG context 8219994
Methylated C's in CHG context 1030273
Methylated C's in CHH context 9468397
Methylated C's in Unknown context 343493
Unmethylated C's in CpG context 43793710
Unmethylated C's in CHG context 72295531
Unmethylated C's in CHH context 233495507
Unmethylated C's in Unknown context 2399471
Percentage methylation (CpG context) 15.8%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3991436 original top strand
CTOT 3145292 complementary to original top strand
CTOB 3103611 complementary to original bottom strand
OB 3936940 original bottom strand

Deduplication

Alignments analysed 14177279
Unique alignments 10805324
Duplicates removed 3371955
Duplicated alignments were found at 2546601 different positions

Cytosine Methylation after Extraction

Total C's analysed 146553907
Methylated C's in CpG context 3242330
Methylated C's in CHG context 415358
Methylated C's in CHH context 3670331
Unmethylated C's in CpG context 17384380
Unmethylated C's in CHG context 28665884
Unmethylated C's in CHH context 93175624
Percentage methylation (CpG context) 15.7%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 3.8%

M-Bias Plot