Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF01-EM01-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF01-EM01-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 47718725
Paired-end alignments with a unique best hit 11837827
Pairs without alignments under any condition 30005679
Pairs that did not map uniquely 5875219
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 315419211
Methylated C's in CpG context 10028002
Methylated C's in CHG context 2595448
Methylated C's in CHH context 15258811
Methylated C's in Unknown context 341029
Unmethylated C's in CpG context 30898045
Unmethylated C's in CHG context 58865061
Unmethylated C's in CHH context 197773844
Unmethylated C's in Unknown context 1525870
Percentage methylation (CpG context) 24.5%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 7.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3036089 original top strand
CTOT 2904525 complementary to original top strand
CTOB 2881668 complementary to original bottom strand
OB 3015542 original bottom strand

Deduplication

Alignments analysed 11837824
Unique alignments 8908927
Duplicates removed 2928897
Duplicated alignments were found at 2175783 different positions

Cytosine Methylation after Extraction

Total C's analysed 131551797
Methylated C's in CpG context 4206137
Methylated C's in CHG context 1155035
Methylated C's in CHH context 6414974
Unmethylated C's in CpG context 12856711
Unmethylated C's in CHG context 24410780
Unmethylated C's in CHH context 82508160
Percentage methylation (CpG context) 24.7%
Percentage methylation (CHG context) 4.5%
Percentage methylation (CHH context) 7.2%

M-Bias Plot