Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM05-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM05-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 63057346
Paired-end alignments with a unique best hit 1698854
Pairs without alignments under any condition 60469188
Pairs that did not map uniquely 889304
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 53131425
Methylated C's in CpG context 968715
Methylated C's in CHG context 154154
Methylated C's in CHH context 2898885
Methylated C's in Unknown context 86455
Unmethylated C's in CpG context 5085353
Unmethylated C's in CHG context 8738717
Unmethylated C's in CHH context 35285601
Unmethylated C's in Unknown context 270113
Percentage methylation (CpG context) 16.0%
Percentage methylation (CHG context) 1.7%
Percentage methylation (CHH context) 7.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 435646 original top strand
CTOT 412268 complementary to original top strand
CTOB 413487 complementary to original bottom strand
OB 437453 original bottom strand

Deduplication

Alignments analysed 1698854
Unique alignments 289989
Duplicates removed 1408865
Duplicated alignments were found at 198364 different positions

Cytosine Methylation after Extraction

Total C's analysed 5867837
Methylated C's in CpG context 95018
Methylated C's in CHG context 18261
Methylated C's in CHH context 373566
Unmethylated C's in CpG context 537095
Unmethylated C's in CHG context 898809
Unmethylated C's in CHH context 3945088
Percentage methylation (CpG context) 15.0%
Percentage methylation (CHG context) 2.0%
Percentage methylation (CHH context) 8.7%

M-Bias Plot