Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM04-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM04-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 54708068
Paired-end alignments with a unique best hit 1245366
Pairs without alignments under any condition 52428088
Pairs that did not map uniquely 1034614
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24523955
Methylated C's in CpG context 385592
Methylated C's in CHG context 141132
Methylated C's in CHH context 5068731
Methylated C's in Unknown context 135348
Unmethylated C's in CpG context 1624565
Unmethylated C's in CHG context 2870352
Unmethylated C's in CHH context 14433583
Unmethylated C's in Unknown context 189745
Percentage methylation (CpG context) 19.2%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 26.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 320163 original top strand
CTOT 301141 complementary to original top strand
CTOB 304639 complementary to original bottom strand
OB 319423 original bottom strand

Deduplication

Alignments analysed 1245366
Unique alignments 685089
Duplicates removed 560277
Duplicated alignments were found at 283238 different positions

Cytosine Methylation after Extraction

Total C's analysed 8071590
Methylated C's in CpG context 126606
Methylated C's in CHG context 39664
Methylated C's in CHH context 1494281
Unmethylated C's in CpG context 558446
Unmethylated C's in CHG context 977524
Unmethylated C's in CHH context 4875069
Percentage methylation (CpG context) 18.5%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 23.5%

M-Bias Plot