Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM03-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM03-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 66096319 Number of paired-end alignments with a unique best hit: 13081605 Mapping efficiency: 19.8% Sequence pairs with no alignments under any condition: 45148855 Sequence pairs did not map uniquely: 7865859 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3802759 ((converted) top strand) GA/CT/CT: 2760336 (complementary to (converted) top strand) GA/CT/GA: 2739636 (complementary to (converted) bottom strand) CT/GA/GA: 3778869 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 264045006 Total methylated C's in CpG context: 5688845 Total methylated C's in CHG context: 1263257 Total methylated C's in CHH context: 13624378 Total methylated C's in Unknown context: 301984 Total unmethylated C's in CpG context: 23292990 Total unmethylated C's in CHG context: 42350005 Total unmethylated C's in CHH context: 177825531 Total unmethylated C's in Unknown context: 1586693 C methylated in CpG context: 19.6% C methylated in CHG context: 2.9% C methylated in CHH context: 7.1% C methylated in unknown context (CN or CHN): 16.0% Bismark completed in 0d 2h 31m 28s