Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM03-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF08-CM03-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 66096319
Paired-end alignments with a unique best hit 13081605
Pairs without alignments under any condition 45148855
Pairs that did not map uniquely 7865859
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 264045006
Methylated C's in CpG context 5688845
Methylated C's in CHG context 1263257
Methylated C's in CHH context 13624378
Methylated C's in Unknown context 301984
Unmethylated C's in CpG context 23292990
Unmethylated C's in CHG context 42350005
Unmethylated C's in CHH context 177825531
Unmethylated C's in Unknown context 1586693
Percentage methylation (CpG context) 19.6%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 7.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3802759 original top strand
CTOT 2760336 complementary to original top strand
CTOB 2739636 complementary to original bottom strand
OB 3778869 original bottom strand

Deduplication

Alignments analysed 13081600
Unique alignments 8968377
Duplicates removed 4113223
Duplicated alignments were found at 2714106 different positions

Cytosine Methylation after Extraction

Total C's analysed 97330064
Methylated C's in CpG context 2079031
Methylated C's in CHG context 465841
Methylated C's in CHH context 4616696
Unmethylated C's in CpG context 8634806
Unmethylated C's in CHG context 15532345
Unmethylated C's in CHH context 66001345
Percentage methylation (CpG context) 19.4%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 6.5%

M-Bias Plot