Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF06-CM01-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF06-CM01-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 82389335
Paired-end alignments with a unique best hit 21391013
Pairs without alignments under any condition 49144786
Pairs that did not map uniquely 11853536
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 486705678
Methylated C's in CpG context 13309555
Methylated C's in CHG context 2668182
Methylated C's in CHH context 20162803
Methylated C's in Unknown context 485728
Unmethylated C's in CpG context 47870691
Unmethylated C's in CHG context 87089171
Unmethylated C's in CHH context 315605276
Unmethylated C's in Unknown context 2841411
Percentage methylation (CpG context) 21.8%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 6.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5828753 original top strand
CTOT 4879701 complementary to original top strand
CTOB 4871977 complementary to original bottom strand
OB 5810575 original bottom strand

Deduplication

Alignments analysed 21391006
Unique alignments 16158598
Duplicates removed 5232408
Duplicated alignments were found at 3908491 different positions

Cytosine Methylation after Extraction

Total C's analysed 194982120
Methylated C's in CpG context 5333947
Methylated C's in CHG context 1128807
Methylated C's in CHH context 8044239
Unmethylated C's in CpG context 19188766
Unmethylated C's in CHG context 34739930
Unmethylated C's in CHH context 126546431
Percentage methylation (CpG context) 21.8%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 6.0%

M-Bias Plot