Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM05-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM05-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 73337225
Paired-end alignments with a unique best hit 19456477
Pairs without alignments under any condition 43774341
Pairs that did not map uniquely 10106407
Genomic sequence context not extractable (edges of chromosomes) 8

Cytosine Methylation

Total C's analysed 535123220
Methylated C's in CpG context 15975122
Methylated C's in CHG context 3070671
Methylated C's in CHH context 18435234
Methylated C's in Unknown context 477737
Unmethylated C's in CpG context 57889418
Unmethylated C's in CHG context 104411314
Unmethylated C's in CHH context 335341461
Unmethylated C's in Unknown context 2603235
Percentage methylation (CpG context) 21.6%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 5.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5199710 original top strand
CTOT 4572984 complementary to original top strand
CTOB 4529764 complementary to original bottom strand
OB 5154011 original bottom strand

Deduplication

Alignments analysed 19456469
Unique alignments 14502599
Duplicates removed 4953870
Duplicated alignments were found at 3651118 different positions

Cytosine Methylation after Extraction

Total C's analysed 215761875
Methylated C's in CpG context 6373641
Methylated C's in CHG context 1254343
Methylated C's in CHH context 7390530
Unmethylated C's in CpG context 23242439
Unmethylated C's in CHG context 41816798
Unmethylated C's in CHH context 135684124
Percentage methylation (CpG context) 21.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 5.2%

M-Bias Plot