Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM02-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM02-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 53936905
Paired-end alignments with a unique best hit 19268727
Pairs without alignments under any condition 24852305
Pairs that did not map uniquely 9815873
Genomic sequence context not extractable (edges of chromosomes) 14

Cytosine Methylation

Total C's analysed 632232949
Methylated C's in CpG context 17125121
Methylated C's in CHG context 1631911
Methylated C's in CHH context 9284949
Methylated C's in Unknown context 307839
Unmethylated C's in CpG context 76864129
Unmethylated C's in CHG context 130104233
Unmethylated C's in CHH context 397222606
Unmethylated C's in Unknown context 2612278
Percentage methylation (CpG context) 18.2%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 2.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4540889 original top strand
CTOT 5088529 complementary to original top strand
CTOB 5090569 complementary to original bottom strand
OB 4548726 original bottom strand

Deduplication

Alignments analysed 19268713
Unique alignments 14427604
Duplicates removed 4841109
Duplicated alignments were found at 3614754 different positions

Cytosine Methylation after Extraction

Total C's analysed 261975744
Methylated C's in CpG context 6990826
Methylated C's in CHG context 702897
Methylated C's in CHH context 3935709
Unmethylated C's in CpG context 31782393
Unmethylated C's in CHG context 53652034
Unmethylated C's in CHH context 164911885
Percentage methylation (CpG context) 18.0%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 2.3%

M-Bias Plot