Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF04-CM04-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF04-CM04-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 41746657
Paired-end alignments with a unique best hit 10918281
Pairs without alignments under any condition 25248177
Pairs that did not map uniquely 5580199
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 285262630
Methylated C's in CpG context 7250811
Methylated C's in CHG context 1271829
Methylated C's in CHH context 8826826
Methylated C's in Unknown context 253453
Unmethylated C's in CpG context 29593044
Unmethylated C's in CHG context 53051780
Unmethylated C's in CHH context 185268340
Unmethylated C's in Unknown context 1461119
Percentage methylation (CpG context) 19.7%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 4.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3116973 original top strand
CTOT 2353642 complementary to original top strand
CTOB 2342600 complementary to original bottom strand
OB 3105061 original bottom strand

Deduplication

Alignments analysed 10918276
Unique alignments 8108328
Duplicates removed 2809948
Duplicated alignments were found at 2061434 different positions

Cytosine Methylation after Extraction

Total C's analysed 115725833
Methylated C's in CpG context 2895514
Methylated C's in CHG context 514765
Methylated C's in CHH context 3459335
Unmethylated C's in CpG context 11983878
Unmethylated C's in CHG context 21345620
Unmethylated C's in CHH context 75526721
Percentage methylation (CpG context) 19.5%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 4.4%

M-Bias Plot