Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM03-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM03-Zygote_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 64470157
Paired-end alignments with a unique best hit 16517068
Pairs without alignments under any condition 38359026
Pairs that did not map uniquely 9594063
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 372908976
Methylated C's in CpG context 9796068
Methylated C's in CHG context 1721896
Methylated C's in CHH context 15173231
Methylated C's in Unknown context 374581
Unmethylated C's in CpG context 36640081
Unmethylated C's in CHG context 67610053
Unmethylated C's in CHH context 241967647
Unmethylated C's in Unknown context 1970981
Percentage methylation (CpG context) 21.1%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4608378 original top strand
CTOT 3664120 complementary to original top strand
CTOB 3651795 complementary to original bottom strand
OB 4592768 original bottom strand

Deduplication

Alignments analysed 16517061
Unique alignments 12492794
Duplicates removed 4024267
Duplicated alignments were found at 2929707 different positions

Cytosine Methylation after Extraction

Total C's analysed 150062562
Methylated C's in CpG context 3919996
Methylated C's in CHG context 698297
Methylated C's in CHH context 5713111
Unmethylated C's in CpG context 14815997
Unmethylated C's in CHG context 27156955
Unmethylated C's in CHH context 97758206
Percentage methylation (CpG context) 20.9%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.5%

M-Bias Plot