Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF01-CM02-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF01-CM02-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 70708148
Paired-end alignments with a unique best hit 24971340
Pairs without alignments under any condition 32368850
Pairs that did not map uniquely 13367958
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 761919570
Methylated C's in CpG context 21632951
Methylated C's in CHG context 2849382
Methylated C's in CHH context 15683163
Methylated C's in Unknown context 457724
Unmethylated C's in CpG context 93811696
Unmethylated C's in CHG context 157562798
Unmethylated C's in CHH context 470379580
Unmethylated C's in Unknown context 3211503
Percentage methylation (CpG context) 18.7%
Percentage methylation (CHG context) 1.8%
Percentage methylation (CHH context) 3.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6075606 original top strand
CTOT 6411238 complementary to original top strand
CTOB 6406104 complementary to original bottom strand
OB 6078385 original bottom strand

Deduplication

Alignments analysed 24971333
Unique alignments 19043207
Duplicates removed 5928126
Duplicated alignments were found at 4466783 different positions

Cytosine Methylation after Extraction

Total C's analysed 312429132
Methylated C's in CpG context 8731550
Methylated C's in CHG context 1186609
Methylated C's in CHH context 6444272
Unmethylated C's in CpG context 38345713
Unmethylated C's in CHG context 64329313
Unmethylated C's in CHH context 193391675
Percentage methylation (CpG context) 18.5%
Percentage methylation (CHG context) 1.8%
Percentage methylation (CHH context) 3.2%

M-Bias Plot