Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth8_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth8_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/ecoli-MG1655/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 85174300 Number of paired-end alignments with a unique best hit: 1830106 Mapping efficiency: 2.1% Sequence pairs with no alignments under any condition: 83269883 Sequence pairs did not map uniquely: 74311 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 413048 ((converted) top strand) GA/CT/CT: 503358 (complementary to (converted) top strand) GA/CT/GA: 500356 (complementary to (converted) bottom strand) CT/GA/GA: 413342 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 101987599 Total methylated C's in CpG context: 408609 Total methylated C's in CHG context: 332944 Total methylated C's in CHH context: 674102 Total methylated C's in Unknown context: 60803 Total unmethylated C's in CpG context: 29942775 Total unmethylated C's in CHG context: 25190219 Total unmethylated C's in CHH context: 45438950 Total unmethylated C's in Unknown context: 279460 C methylated in CpG context: 1.3% C methylated in CHG context: 1.3% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 17.9% Bismark completed in 0d 1h 31m 26s