Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth16_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth16_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/ecoli-MG1655/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 73273057 Number of paired-end alignments with a unique best hit: 13777977 Mapping efficiency: 18.8% Sequence pairs with no alignments under any condition: 59159400 Sequence pairs did not map uniquely: 335680 Sequence pairs which were discarded because genomic sequence could not be extracted: 4 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3151304 ((converted) top strand) GA/CT/CT: 3763289 (complementary to (converted) top strand) GA/CT/GA: 3738622 (complementary to (converted) bottom strand) CT/GA/GA: 3124758 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 796084119 Total methylated C's in CpG context: 3069507 Total methylated C's in CHG context: 2629289 Total methylated C's in CHH context: 3997006 Total methylated C's in Unknown context: 498343 Total unmethylated C's in CpG context: 234859593 Total unmethylated C's in CHG context: 197034366 Total unmethylated C's in CHH context: 354494358 Total unmethylated C's in Unknown context: 2188269 C methylated in CpG context: 1.3% C methylated in CHG context: 1.3% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 18.5% Bismark completed in 0d 3h 1m 2s