Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth15_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth15_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/ecoli-MG1655/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 108911247 Number of paired-end alignments with a unique best hit: 9108570 Mapping efficiency: 8.4% Sequence pairs with no alignments under any condition: 99342731 Sequence pairs did not map uniquely: 459946 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2199593 ((converted) top strand) GA/CT/CT: 2351436 (complementary to (converted) top strand) GA/CT/GA: 2355183 (complementary to (converted) bottom strand) CT/GA/GA: 2202358 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 466793413 Total methylated C's in CpG context: 4433012 Total methylated C's in CHG context: 713048 Total methylated C's in CHH context: 1663938 Total methylated C's in Unknown context: 8878 Total unmethylated C's in CpG context: 144957150 Total unmethylated C's in CHG context: 120042295 Total unmethylated C's in CHH context: 194983970 Total unmethylated C's in Unknown context: 48293 C methylated in CpG context: 3.0% C methylated in CHG context: 0.6% C methylated in CHH context: 0.8% C methylated in unknown context (CN or CHN): 15.5% Bismark completed in 0d 2h 43m 48s