Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth11_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth11_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/ecoli-MG1655/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 56845589 Number of paired-end alignments with a unique best hit: 676715 Mapping efficiency: 1.2% Sequence pairs with no alignments under any condition: 56139484 Sequence pairs did not map uniquely: 29390 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 150149 ((converted) top strand) GA/CT/CT: 188915 (complementary to (converted) top strand) GA/CT/GA: 188444 (complementary to (converted) bottom strand) CT/GA/GA: 149207 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 36266037 Total methylated C's in CpG context: 127853 Total methylated C's in CHG context: 109755 Total methylated C's in CHH context: 238440 Total methylated C's in Unknown context: 13844 Total unmethylated C's in CpG context: 10823684 Total unmethylated C's in CHG context: 9040624 Total unmethylated C's in CHH context: 15925681 Total unmethylated C's in Unknown context: 85814 C methylated in CpG context: 1.2% C methylated in CHG context: 1.2% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 13.9% Bismark completed in 0d 0h 56m 28s