Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth7_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth7_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 95537325 Number of paired-end alignments with a unique best hit: 4192 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 95532882 Sequence pairs did not map uniquely: 251 Sequence pairs which were discarded because genomic sequence could not be extracted: 25 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 816 ((converted) top strand) GA/CT/CT: 1170 (complementary to (converted) top strand) GA/CT/GA: 1270 (complementary to (converted) bottom strand) CT/GA/GA: 911 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 132154 Total methylated C's in CpG context: 6461 Total methylated C's in CHG context: 6379 Total methylated C's in CHH context: 15414 Total methylated C's in Unknown context: 49 Total unmethylated C's in CpG context: 24160 Total unmethylated C's in CHG context: 26570 Total unmethylated C's in CHH context: 53170 Total unmethylated C's in Unknown context: 144 C methylated in CpG context: 21.1% C methylated in CHG context: 19.4% C methylated in CHH context: 22.5% C methylated in unknown context (CN or CHN): 25.4% Bismark completed in 0d 1h 25m 56s