Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth14_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth14_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 69159859 Number of paired-end alignments with a unique best hit: 10026 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 69149739 Sequence pairs did not map uniquely: 94 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2999 ((converted) top strand) GA/CT/CT: 2286 (complementary to (converted) top strand) GA/CT/GA: 2352 (complementary to (converted) bottom strand) CT/GA/GA: 2389 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 284707 Total methylated C's in CpG context: 3871 Total methylated C's in CHG context: 1597 Total methylated C's in CHH context: 11774 Total methylated C's in Unknown context: 2 Total unmethylated C's in CpG context: 72347 Total unmethylated C's in CHG context: 80823 Total unmethylated C's in CHH context: 114295 Total unmethylated C's in Unknown context: 171 C methylated in CpG context: 5.1% C methylated in CHG context: 1.9% C methylated in CHH context: 9.3% C methylated in unknown context (CN or CHN): 1.2% Bismark completed in 0d 1h 2m 22s