Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth13_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth13_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100607927 Number of paired-end alignments with a unique best hit: 12691 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 100595116 Sequence pairs did not map uniquely: 120 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3198 ((converted) top strand) GA/CT/CT: 2896 (complementary to (converted) top strand) GA/CT/GA: 3605 (complementary to (converted) bottom strand) CT/GA/GA: 2991 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 400974 Total methylated C's in CpG context: 5932 Total methylated C's in CHG context: 1955 Total methylated C's in CHH context: 11366 Total methylated C's in Unknown context: 10 Total unmethylated C's in CpG context: 103277 Total unmethylated C's in CHG context: 116507 Total unmethylated C's in CHH context: 161937 Total unmethylated C's in Unknown context: 316 C methylated in CpG context: 5.4% C methylated in CHG context: 1.7% C methylated in CHH context: 6.6% C methylated in unknown context (CN or CHN): 3.1% Bismark completed in 0d 1h 31m 8s