Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Meth10_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Meth10_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 83382907 Number of paired-end alignments with a unique best hit: 4575 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 83378138 Sequence pairs did not map uniquely: 194 Sequence pairs which were discarded because genomic sequence could not be extracted: 27 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1100 ((converted) top strand) GA/CT/CT: 1269 (complementary to (converted) top strand) GA/CT/GA: 1181 (complementary to (converted) bottom strand) CT/GA/GA: 998 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 156842 Total methylated C's in CpG context: 767 Total methylated C's in CHG context: 1049 Total methylated C's in CHH context: 6714 Total methylated C's in Unknown context: 22 Total unmethylated C's in CpG context: 36472 Total unmethylated C's in CHG context: 39789 Total unmethylated C's in CHH context: 72051 Total unmethylated C's in Unknown context: 233 C methylated in CpG context: 2.1% C methylated in CHG context: 2.6% C methylated in CHH context: 8.5% C methylated in unknown context (CN or CHN): 8.6% Bismark completed in 0d 1h 15m 37s