Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R2_001_val_2.fq.gz

Data processed at 23:49 on 2020-03-24


Alignment Stats

Sequence pairs analysed in total 24510805
Paired-end alignments with a unique best hit 8593707
Pairs without alignments under any condition 13929047
Pairs that did not map uniquely 1988051
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 251244640
Methylated C's in CpG context 9311185
Methylated C's in CHG context 1241250
Methylated C's in CHH context 3334595
Methylated C's in Unknown context 63649
Unmethylated C's in CpG context 25878911
Unmethylated C's in CHG context 52710364
Unmethylated C's in CHH context 158768335
Unmethylated C's in Unknown context 546831
Percentage methylation (CpG context) 26.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 2.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4237007 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4356698 original bottom strand

Deduplication

Alignments analysed 8593705
Unique alignments 6330826
Duplicates removed 2262879
Duplicated alignments were found at 1443182 different positions

Cytosine Methylation after Extraction

Total C's analysed 124508835
Methylated C's in CpG context 4903316
Methylated C's in CHG context 619102
Methylated C's in CHH context 1795108
Unmethylated C's in CpG context 12097133
Unmethylated C's in CHG context 25334277
Unmethylated C's in CHH context 79759899
Percentage methylation (CpG context) 28.8%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 2.2%

M-Bias Plot