Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-145_S38_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-145_S38_L005_R2_001_val_2.fq.gz

Data processed at 23:49 on 2020-03-24


Alignment Stats

Sequence pairs analysed in total 25314513
Paired-end alignments with a unique best hit 11191276
Pairs without alignments under any condition 11992514
Pairs that did not map uniquely 2130723
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 346932784
Methylated C's in CpG context 12322055
Methylated C's in CHG context 1693840
Methylated C's in CHH context 2773795
Methylated C's in Unknown context 48529
Unmethylated C's in CpG context 36498527
Unmethylated C's in CHG context 70595301
Unmethylated C's in CHH context 223049266
Unmethylated C's in Unknown context 722709
Percentage methylation (CpG context) 25.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5539947 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5651327 original bottom strand

Deduplication

Alignments analysed 11191274
Unique alignments 9355933
Duplicates removed 1835341
Duplicated alignments were found at 997702 different positions

Cytosine Methylation after Extraction

Total C's analysed 214985786
Methylated C's in CpG context 8245428
Methylated C's in CHG context 1047240
Methylated C's in CHH context 1811240
Unmethylated C's in CpG context 21238566
Unmethylated C's in CHG context 42806098
Unmethylated C's in CHH context 139837214
Percentage methylation (CpG context) 28.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.3%

M-Bias Plot