Bismark report for: /gscratch/scrubbed/strigg/analyses/20200319/specify_a/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200319/specify_a/EPI-167_S10_L002_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v01/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 24340737 Number of paired-end alignments with a unique best hit: 10282831 Mapping efficiency: 42.2% Sequence pairs with no alignments under any condition: 11547203 Sequence pairs did not map uniquely: 2510703 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5109279 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5173545 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 276968626 Total methylated C's in CpG context: 8922644 Total methylated C's in CHG context: 999800 Total methylated C's in CHH context: 4718477 Total methylated C's in Unknown context: 31009 Total unmethylated C's in CpG context: 25955060 Total unmethylated C's in CHG context: 56364634 Total unmethylated C's in CHH context: 180008011 Total unmethylated C's in Unknown context: 574541 C methylated in CpG context: 25.6% C methylated in CHG context: 1.7% C methylated in CHH context: 2.6% C methylated in unknown context (CN or CHN): 5.1% Bismark completed in 0d 0h 55m 53s