Bismark

Bismark Processing Report

/gscratch/scrubbed/strigg/analyses/20200319/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200319/EPI-167_S10_L002_R2_001_val_2.fq.gz

Data processed at 21:17 on 2020-03-19

Alignment Stats

Sequence pairs analysed in total 24337012
Paired-end alignments with a unique best hit 10281806
Pairs without alignments under any condition 11544916
Pairs that did not map uniquely 2510290
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 276946851
Methylated C's in CpG context 8921824
Methylated C's in CHG context 999580
Methylated C's in CHH context 4717727
Methylated C's in Unknown context 30868
Unmethylated C's in CpG context 25953298
Unmethylated C's in CHG context 56361217
Unmethylated C's in CHH context 179993205
Unmethylated C's in Unknown context 574493
Percentage methylation (CpG context) 25.6%
Percentage methylation (CHG context) 1.7%
Percentage methylation (CHH context) 2.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5108752 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5173047 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 204982789
Methylated C's in CpG context 6641793
Methylated C's in CHG context 730293
Methylated C's in CHH context 3398833
Unmethylated C's in CpG context 19166987
Unmethylated C's in CHG context 41256598
Unmethylated C's in CHH context 133788285
Percentage methylation (CpG context) 25.7%
Percentage methylation (CHG context) 1.7%
Percentage methylation (CHH context) 2.5%

M-Bias Plot