Bismark

Bismark Processing Report

/gscratch/scrubbed/strigg/analyses/20200319/specify_a/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200319/specify_a/EPI-167_S10_L002_R2_001_val_2.fq.gz

Data processed at 19:56 on 2020-03-19

Alignment Stats

Sequence pairs analysed in total 24340737
Paired-end alignments with a unique best hit 10282831
Pairs without alignments under any condition 11547203
Pairs that did not map uniquely 2510703
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 276968626
Methylated C's in CpG context 8922644
Methylated C's in CHG context 999800
Methylated C's in CHH context 4718477
Methylated C's in Unknown context 31009
Unmethylated C's in CpG context 25955060
Unmethylated C's in CHG context 56364634
Unmethylated C's in CHH context 180008011
Unmethylated C's in Unknown context 574541
Percentage methylation (CpG context) 25.6%
Percentage methylation (CHG context) 1.7%
Percentage methylation (CHH context) 2.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5109279 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5173545 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 204991388
Methylated C's in CpG context 6642017
Methylated C's in CHG context 730181
Methylated C's in CHH context 3398529
Unmethylated C's in CpG context 19167685
Unmethylated C's in CHG context 41257959
Unmethylated C's in CHH context 133795017
Percentage methylation (CpG context) 25.7%
Percentage methylation (CHG context) 1.7%
Percentage methylation (CHH context) 2.5%

M-Bias Plot