Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth9_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth9_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 87033711 Number of paired-end alignments with a unique best hit: 28863167 Mapping efficiency: 33.2% Sequence pairs with no alignments under any condition: 54666408 Sequence pairs did not map uniquely: 3504136 Sequence pairs which were discarded because genomic sequence could not be extracted: 156254 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7044665 ((converted) top strand) GA/CT/CT: 7278804 (complementary to (converted) top strand) GA/CT/GA: 7325794 (complementary to (converted) bottom strand) CT/GA/GA: 7057650 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1185366396 Total methylated C's in CpG context: 34429781 Total methylated C's in CHG context: 3309395 Total methylated C's in CHH context: 12648356 Total methylated C's in Unknown context: 827057 Total unmethylated C's in CpG context: 133513261 Total unmethylated C's in CHG context: 216384943 Total unmethylated C's in CHH context: 785080660 Total unmethylated C's in Unknown context: 4659006 C methylated in CpG context: 20.5% C methylated in CHG context: 1.5% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 15.1% Bismark completed in 0d 5h 15m 2s