Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth5_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth5_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 284116370 Number of paired-end alignments with a unique best hit: 176448073 Mapping efficiency: 62.1% Sequence pairs with no alignments under any condition: 74938987 Sequence pairs did not map uniquely: 32729310 Sequence pairs which were discarded because genomic sequence could not be extracted: 1476881 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 41946437 ((converted) top strand) GA/CT/CT: 45593288 (complementary to (converted) top strand) GA/CT/GA: 45674173 (complementary to (converted) bottom strand) CT/GA/GA: 41757294 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 8047605825 Total methylated C's in CpG context: 80058040 Total methylated C's in CHG context: 12048677 Total methylated C's in CHH context: 34553198 Total methylated C's in Unknown context: 628388 Total unmethylated C's in CpG context: 1691019586 Total unmethylated C's in CHG context: 1586289138 Total unmethylated C's in CHH context: 4643637186 Total unmethylated C's in Unknown context: 14307844 C methylated in CpG context: 4.5% C methylated in CHG context: 0.8% C methylated in CHH context: 0.7% C methylated in unknown context (CN or CHN): 4.2% Bismark completed in 1d 3h 28m 50s