Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth4_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth4_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 149828789 Number of paired-end alignments with a unique best hit: 88645190 Mapping efficiency: 59.2% Sequence pairs with no alignments under any condition: 44733422 Sequence pairs did not map uniquely: 16450177 Sequence pairs which were discarded because genomic sequence could not be extracted: 946133 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 21348560 ((converted) top strand) GA/CT/CT: 22757950 (complementary to (converted) top strand) GA/CT/GA: 22595986 (complementary to (converted) bottom strand) CT/GA/GA: 20996561 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3982571955 Total methylated C's in CpG context: 45227861 Total methylated C's in CHG context: 7924621 Total methylated C's in CHH context: 23951778 Total methylated C's in Unknown context: 392267 Total unmethylated C's in CpG context: 832099269 Total unmethylated C's in CHG context: 784473522 Total unmethylated C's in CHH context: 2288894904 Total unmethylated C's in Unknown context: 7106292 C methylated in CpG context: 5.2% C methylated in CHG context: 1.0% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 5.2% Bismark completed in 0d 14h 1m 29s