Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth3_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth3_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 77672273 Number of paired-end alignments with a unique best hit: 36844251 Mapping efficiency: 47.4% Sequence pairs with no alignments under any condition: 37406848 Sequence pairs did not map uniquely: 3421174 Sequence pairs which were discarded because genomic sequence could not be extracted: 152406 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8721859 ((converted) top strand) GA/CT/CT: 9579752 (complementary to (converted) top strand) GA/CT/GA: 9627318 (complementary to (converted) bottom strand) CT/GA/GA: 8762916 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1548334108 Total methylated C's in CpG context: 7964851 Total methylated C's in CHG context: 3082589 Total methylated C's in CHH context: 11703888 Total methylated C's in Unknown context: 696538 Total unmethylated C's in CpG context: 231556246 Total unmethylated C's in CHG context: 277818283 Total unmethylated C's in CHH context: 1016208251 Total unmethylated C's in Unknown context: 5382089 C methylated in CpG context: 3.3% C methylated in CHG context: 1.1% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 11.5% Bismark completed in 0d 6h 21m 13s