Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth2_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth2_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 84959218 Number of paired-end alignments with a unique best hit: 43721874 Mapping efficiency: 51.5% Sequence pairs with no alignments under any condition: 36762317 Sequence pairs did not map uniquely: 4475027 Sequence pairs which were discarded because genomic sequence could not be extracted: 175376 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10205270 ((converted) top strand) GA/CT/CT: 11514319 (complementary to (converted) top strand) GA/CT/GA: 11577108 (complementary to (converted) bottom strand) CT/GA/GA: 10249801 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1803315382 Total methylated C's in CpG context: 8199601 Total methylated C's in CHG context: 3642279 Total methylated C's in CHH context: 14139495 Total methylated C's in Unknown context: 715826 Total unmethylated C's in CpG context: 270063644 Total unmethylated C's in CHG context: 324028897 Total unmethylated C's in CHH context: 1183241466 Total unmethylated C's in Unknown context: 5890228 C methylated in CpG context: 2.9% C methylated in CHG context: 1.1% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 10.8% Bismark completed in 0d 7h 19m 27s