Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth1_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Pact_trim/Meth1_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 74424695 Number of paired-end alignments with a unique best hit: 36318677 Mapping efficiency: 48.8% Sequence pairs with no alignments under any condition: 34799849 Sequence pairs did not map uniquely: 3306169 Sequence pairs which were discarded because genomic sequence could not be extracted: 149828 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8474565 ((converted) top strand) GA/CT/CT: 9561246 (complementary to (converted) top strand) GA/CT/GA: 9620604 (complementary to (converted) bottom strand) CT/GA/GA: 8512434 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1538382421 Total methylated C's in CpG context: 7394976 Total methylated C's in CHG context: 3021456 Total methylated C's in CHH context: 11434246 Total methylated C's in Unknown context: 676167 Total unmethylated C's in CpG context: 227923669 Total unmethylated C's in CHG context: 273965285 Total unmethylated C's in CHH context: 1014642789 Total unmethylated C's in Unknown context: 5141199 C methylated in CpG context: 3.1% C methylated in CHG context: 1.1% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 11.6% Bismark completed in 0d 6h 11m 17s