Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth18_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth18_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 61585400 Number of paired-end alignments with a unique best hit: 14001402 Mapping efficiency: 22.7% Sequence pairs with no alignments under any condition: 41193946 Sequence pairs did not map uniquely: 6390052 Sequence pairs which were discarded because genomic sequence could not be extracted: 221 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3447824 ((converted) top strand) GA/CT/CT: 3561023 (complementary to (converted) top strand) GA/CT/GA: 3549170 (complementary to (converted) bottom strand) CT/GA/GA: 3443164 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 631032858 Total methylated C's in CpG context: 14510651 Total methylated C's in CHG context: 1426130 Total methylated C's in CHH context: 5563948 Total methylated C's in Unknown context: 437143 Total unmethylated C's in CpG context: 92393953 Total unmethylated C's in CHG context: 109898929 Total unmethylated C's in CHH context: 407239247 Total unmethylated C's in Unknown context: 1936782 C methylated in CpG context: 13.6% C methylated in CHG context: 1.3% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 18.4% Bismark completed in 0d 3h 11m 57s