Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth14_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth14_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 69159859 Number of paired-end alignments with a unique best hit: 36472079 Mapping efficiency: 52.7% Sequence pairs with no alignments under any condition: 10355216 Sequence pairs did not map uniquely: 22332564 Sequence pairs which were discarded because genomic sequence could not be extracted: 168 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9986376 ((converted) top strand) GA/CT/CT: 8265281 (complementary to (converted) top strand) GA/CT/GA: 8242390 (complementary to (converted) bottom strand) CT/GA/GA: 9977864 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1719504853 Total methylated C's in CpG context: 23193334 Total methylated C's in CHG context: 2190014 Total methylated C's in CHH context: 6278238 Total methylated C's in Unknown context: 64206 Total unmethylated C's in CpG context: 347752341 Total unmethylated C's in CHG context: 325108689 Total unmethylated C's in CHH context: 1014982237 Total unmethylated C's in Unknown context: 1766897 C methylated in CpG context: 6.3% C methylated in CHG context: 0.7% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 3.5% Bismark completed in 0d 6h 18m 18s