Bismark report for: /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth10_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031520-TG-bs/Mcap_trim/Meth10_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 83382907 Number of paired-end alignments with a unique best hit: 33024348 Mapping efficiency: 39.6% Sequence pairs with no alignments under any condition: 35534143 Sequence pairs did not map uniquely: 14824416 Sequence pairs which were discarded because genomic sequence could not be extracted: 563 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7768041 ((converted) top strand) GA/CT/CT: 8745272 (complementary to (converted) top strand) GA/CT/GA: 8753450 (complementary to (converted) bottom strand) CT/GA/GA: 7757022 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1475090477 Total methylated C's in CpG context: 29844699 Total methylated C's in CHG context: 3296608 Total methylated C's in CHH context: 11774846 Total methylated C's in Unknown context: 800724 Total unmethylated C's in CpG context: 217800127 Total unmethylated C's in CHG context: 258991853 Total unmethylated C's in CHH context: 953382344 Total unmethylated C's in Unknown context: 4438005 C methylated in CpG context: 12.1% C methylated in CHG context: 1.3% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 15.3% Bismark completed in 0d 6h 9m 36s