Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth18_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth18_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 2278352 Mapping efficiency: 22.8% Sequence pairs with no alignments under any condition: 6682550 Sequence pairs did not map uniquely: 1039098 Sequence pairs which were discarded because genomic sequence could not be extracted: 35 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 561403 ((converted) top strand) GA/CT/CT: 579475 (complementary to (converted) top strand) GA/CT/GA: 577710 (complementary to (converted) bottom strand) CT/GA/GA: 559729 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 102720230 Total methylated C's in CpG context: 2365050 Total methylated C's in CHG context: 233329 Total methylated C's in CHH context: 904159 Total methylated C's in Unknown context: 71651 Total unmethylated C's in CpG context: 15039802 Total unmethylated C's in CHG context: 17887150 Total unmethylated C's in CHH context: 66290740 Total unmethylated C's in Unknown context: 314958 C methylated in CpG context: 13.6% C methylated in CHG context: 1.3% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 18.5% Bismark completed in 0d 0h 23m 46s