Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth17_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth17_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 2352210 Mapping efficiency: 23.5% Sequence pairs with no alignments under any condition: 6441804 Sequence pairs did not map uniquely: 1205986 Sequence pairs which were discarded because genomic sequence could not be extracted: 45 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 579145 ((converted) top strand) GA/CT/CT: 597245 (complementary to (converted) top strand) GA/CT/GA: 596824 (complementary to (converted) bottom strand) CT/GA/GA: 578951 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 102273667 Total methylated C's in CpG context: 2459753 Total methylated C's in CHG context: 234880 Total methylated C's in CHH context: 911970 Total methylated C's in Unknown context: 71040 Total unmethylated C's in CpG context: 15029235 Total unmethylated C's in CHG context: 17865122 Total unmethylated C's in CHH context: 65772707 Total unmethylated C's in Unknown context: 332125 C methylated in CpG context: 14.1% C methylated in CHG context: 1.3% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 17.6% Bismark completed in 0d 0h 24m 5s