Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth16_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth16_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 2432140 Mapping efficiency: 24.3% Sequence pairs with no alignments under any condition: 6399595 Sequence pairs did not map uniquely: 1168265 Sequence pairs which were discarded because genomic sequence could not be extracted: 52 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 603496 ((converted) top strand) GA/CT/CT: 614061 (complementary to (converted) top strand) GA/CT/GA: 612388 (complementary to (converted) bottom strand) CT/GA/GA: 602143 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 109327196 Total methylated C's in CpG context: 2848838 Total methylated C's in CHG context: 261374 Total methylated C's in CHH context: 987228 Total methylated C's in Unknown context: 82280 Total unmethylated C's in CpG context: 15471933 Total unmethylated C's in CHG context: 19009241 Total unmethylated C's in CHH context: 70748582 Total unmethylated C's in Unknown context: 348986 C methylated in CpG context: 15.5% C methylated in CHG context: 1.4% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 19.1% Bismark completed in 0d 0h 24m 24s